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rat renal tubule epithelial cells nrk52e  (Procell Inc)

 
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    Procell Inc rat renal tubule epithelial cells nrk52e
    Claudin-2 deficiency induced tubular <t>epithelial</t> CTGF is involved in fibroblast activation and proliferation. ( A and B ) Immunocytochemistry revealed the nuclear localization of proliferating cell nuclear antigen (PCNA) in activated-fibroblasts in 5.5mM D-glucose (NG) medium or 30mM D-glucose (HG) medium ± co-culture with <t>NRK52E</t> cells ± si- Cldn2 ± pcDNA3.1- Cldn2 ± si- Ctgf ± pcDNA3.1- Ctgf . Cells were immune-stained for α-SMA (red) and PCNA (green) and counterstained with DAPI (blue). White arrows indicate positive staining. Magnification ×400, scale bar =100µm. ( C and D ) Representative image and quantitative analysis show the effect of interfering with Claudin-2 and CTGF protein expression in PTECs on fibroblast proliferation in 5.5mM D-glucose (NG) medium or 30mM D-glucose (HG) medium. Data are the mean ± SEM (n=3). ** P <0.01, vs NRK52E-si- Cldn2 -si-NC+NRK49F+NG; ## P <0.01, vs NRK52E-pcDNA3.1- Cldn2 -pcDNA3.1-NC+HG.
    Rat Renal Tubule Epithelial Cells Nrk52e, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat renal tubule epithelial cells nrk52e/product/Procell Inc
    Average 90 stars, based on 1 article reviews
    rat renal tubule epithelial cells nrk52e - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Claudin-2 Mediates the Proximal Tubular Epithelial Cell–Fibroblast Crosstalk via Paracrine CTGF"

    Article Title: Claudin-2 Mediates the Proximal Tubular Epithelial Cell–Fibroblast Crosstalk via Paracrine CTGF

    Journal: Diabetes, Metabolic Syndrome and Obesity

    doi: 10.2147/DMSO.S432173

    Claudin-2 deficiency induced tubular epithelial CTGF is involved in fibroblast activation and proliferation. ( A and B ) Immunocytochemistry revealed the nuclear localization of proliferating cell nuclear antigen (PCNA) in activated-fibroblasts in 5.5mM D-glucose (NG) medium or 30mM D-glucose (HG) medium ± co-culture with NRK52E cells ± si- Cldn2 ± pcDNA3.1- Cldn2 ± si- Ctgf ± pcDNA3.1- Ctgf . Cells were immune-stained for α-SMA (red) and PCNA (green) and counterstained with DAPI (blue). White arrows indicate positive staining. Magnification ×400, scale bar =100µm. ( C and D ) Representative image and quantitative analysis show the effect of interfering with Claudin-2 and CTGF protein expression in PTECs on fibroblast proliferation in 5.5mM D-glucose (NG) medium or 30mM D-glucose (HG) medium. Data are the mean ± SEM (n=3). ** P <0.01, vs NRK52E-si- Cldn2 -si-NC+NRK49F+NG; ## P <0.01, vs NRK52E-pcDNA3.1- Cldn2 -pcDNA3.1-NC+HG.
    Figure Legend Snippet: Claudin-2 deficiency induced tubular epithelial CTGF is involved in fibroblast activation and proliferation. ( A and B ) Immunocytochemistry revealed the nuclear localization of proliferating cell nuclear antigen (PCNA) in activated-fibroblasts in 5.5mM D-glucose (NG) medium or 30mM D-glucose (HG) medium ± co-culture with NRK52E cells ± si- Cldn2 ± pcDNA3.1- Cldn2 ± si- Ctgf ± pcDNA3.1- Ctgf . Cells were immune-stained for α-SMA (red) and PCNA (green) and counterstained with DAPI (blue). White arrows indicate positive staining. Magnification ×400, scale bar =100µm. ( C and D ) Representative image and quantitative analysis show the effect of interfering with Claudin-2 and CTGF protein expression in PTECs on fibroblast proliferation in 5.5mM D-glucose (NG) medium or 30mM D-glucose (HG) medium. Data are the mean ± SEM (n=3). ** P <0.01, vs NRK52E-si- Cldn2 -si-NC+NRK49F+NG; ## P <0.01, vs NRK52E-pcDNA3.1- Cldn2 -pcDNA3.1-NC+HG.

    Techniques Used: Activation Assay, Immunocytochemistry, Co-Culture Assay, Staining, Expressing

    Claudin-2 deficiency induced tubular epithelial CTGF facilitates fibroblasts to product matrix protein. ( A ) Western blot demonstrated CTGF inhibition in NRK52E-si- Cldn2 cells decreased α-SMA and collage I expression in NRK49F cells, when the cells were co-cultured in 5.5mM D-glucose (NG) medium. Overexpression of CTGF in NRK52E-pcDNA3.1- Cldn2 cells increased α-SMA and collage I expression in NRK49F cells, when the cells were co-cultured in 30mM D-glucose (HG) medium. ( B and C ) The interference efficiency of Claudin-2 and CTGF protein expression in NRK52E cells were confirmed by Western blot analyses. Quantitative analysis of Claudin-2 and CTGF are shown above. ( D and E ) Quantitative analysis of α-SMA and collagen I in NRK49F cells are shown above. Data are expressed as mean data ± SEM (n = 3), ** P <0.01, NRK52E-si- Cldn2 -si-NC+NRK49F+NG; ## P <0.01, vs NRK52E-pcDNA3.1- Cldn2 -pcDNA3.1-NC+HG.
    Figure Legend Snippet: Claudin-2 deficiency induced tubular epithelial CTGF facilitates fibroblasts to product matrix protein. ( A ) Western blot demonstrated CTGF inhibition in NRK52E-si- Cldn2 cells decreased α-SMA and collage I expression in NRK49F cells, when the cells were co-cultured in 5.5mM D-glucose (NG) medium. Overexpression of CTGF in NRK52E-pcDNA3.1- Cldn2 cells increased α-SMA and collage I expression in NRK49F cells, when the cells were co-cultured in 30mM D-glucose (HG) medium. ( B and C ) The interference efficiency of Claudin-2 and CTGF protein expression in NRK52E cells were confirmed by Western blot analyses. Quantitative analysis of Claudin-2 and CTGF are shown above. ( D and E ) Quantitative analysis of α-SMA and collagen I in NRK49F cells are shown above. Data are expressed as mean data ± SEM (n = 3), ** P <0.01, NRK52E-si- Cldn2 -si-NC+NRK49F+NG; ## P <0.01, vs NRK52E-pcDNA3.1- Cldn2 -pcDNA3.1-NC+HG.

    Techniques Used: Western Blot, Inhibition, Expressing, Cell Culture, Over Expression



    Similar Products

    rat renal tubule epithelial cells nrk52e  (Procell Inc)
    90
    Procell Inc rat renal tubule epithelial cells nrk52e
    Claudin-2 deficiency induced tubular <t>epithelial</t> CTGF is involved in fibroblast activation and proliferation. ( A and B ) Immunocytochemistry revealed the nuclear localization of proliferating cell nuclear antigen (PCNA) in activated-fibroblasts in 5.5mM D-glucose (NG) medium or 30mM D-glucose (HG) medium ± co-culture with <t>NRK52E</t> cells ± si- Cldn2 ± pcDNA3.1- Cldn2 ± si- Ctgf ± pcDNA3.1- Ctgf . Cells were immune-stained for α-SMA (red) and PCNA (green) and counterstained with DAPI (blue). White arrows indicate positive staining. Magnification ×400, scale bar =100µm. ( C and D ) Representative image and quantitative analysis show the effect of interfering with Claudin-2 and CTGF protein expression in PTECs on fibroblast proliferation in 5.5mM D-glucose (NG) medium or 30mM D-glucose (HG) medium. Data are the mean ± SEM (n=3). ** P <0.01, vs NRK52E-si- Cldn2 -si-NC+NRK49F+NG; ## P <0.01, vs NRK52E-pcDNA3.1- Cldn2 -pcDNA3.1-NC+HG.
    Rat Renal Tubule Epithelial Cells Nrk52e, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat renal tubule epithelial cells nrk52e/product/Procell Inc
    Average 90 stars, based on 1 article reviews
    rat renal tubule epithelial cells nrk52e - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Claudin-2 deficiency induced tubular epithelial CTGF is involved in fibroblast activation and proliferation. ( A and B ) Immunocytochemistry revealed the nuclear localization of proliferating cell nuclear antigen (PCNA) in activated-fibroblasts in 5.5mM D-glucose (NG) medium or 30mM D-glucose (HG) medium ± co-culture with NRK52E cells ± si- Cldn2 ± pcDNA3.1- Cldn2 ± si- Ctgf ± pcDNA3.1- Ctgf . Cells were immune-stained for α-SMA (red) and PCNA (green) and counterstained with DAPI (blue). White arrows indicate positive staining. Magnification ×400, scale bar =100µm. ( C and D ) Representative image and quantitative analysis show the effect of interfering with Claudin-2 and CTGF protein expression in PTECs on fibroblast proliferation in 5.5mM D-glucose (NG) medium or 30mM D-glucose (HG) medium. Data are the mean ± SEM (n=3). ** P <0.01, vs NRK52E-si- Cldn2 -si-NC+NRK49F+NG; ## P <0.01, vs NRK52E-pcDNA3.1- Cldn2 -pcDNA3.1-NC+HG.

    Journal: Diabetes, Metabolic Syndrome and Obesity

    Article Title: Claudin-2 Mediates the Proximal Tubular Epithelial Cell–Fibroblast Crosstalk via Paracrine CTGF

    doi: 10.2147/DMSO.S432173

    Figure Lengend Snippet: Claudin-2 deficiency induced tubular epithelial CTGF is involved in fibroblast activation and proliferation. ( A and B ) Immunocytochemistry revealed the nuclear localization of proliferating cell nuclear antigen (PCNA) in activated-fibroblasts in 5.5mM D-glucose (NG) medium or 30mM D-glucose (HG) medium ± co-culture with NRK52E cells ± si- Cldn2 ± pcDNA3.1- Cldn2 ± si- Ctgf ± pcDNA3.1- Ctgf . Cells were immune-stained for α-SMA (red) and PCNA (green) and counterstained with DAPI (blue). White arrows indicate positive staining. Magnification ×400, scale bar =100µm. ( C and D ) Representative image and quantitative analysis show the effect of interfering with Claudin-2 and CTGF protein expression in PTECs on fibroblast proliferation in 5.5mM D-glucose (NG) medium or 30mM D-glucose (HG) medium. Data are the mean ± SEM (n=3). ** P <0.01, vs NRK52E-si- Cldn2 -si-NC+NRK49F+NG; ## P <0.01, vs NRK52E-pcDNA3.1- Cldn2 -pcDNA3.1-NC+HG.

    Article Snippet: The normal rat kidney fibroblast cell (NRK49F) and rat renal tubule epithelial cells (NRK52E) were obtained from ProCell Corporation (Wuhan, China) and cultured in RPMI-1640 medium (Corning, NY, USA).

    Techniques: Activation Assay, Immunocytochemistry, Co-Culture Assay, Staining, Expressing

    Claudin-2 deficiency induced tubular epithelial CTGF facilitates fibroblasts to product matrix protein. ( A ) Western blot demonstrated CTGF inhibition in NRK52E-si- Cldn2 cells decreased α-SMA and collage I expression in NRK49F cells, when the cells were co-cultured in 5.5mM D-glucose (NG) medium. Overexpression of CTGF in NRK52E-pcDNA3.1- Cldn2 cells increased α-SMA and collage I expression in NRK49F cells, when the cells were co-cultured in 30mM D-glucose (HG) medium. ( B and C ) The interference efficiency of Claudin-2 and CTGF protein expression in NRK52E cells were confirmed by Western blot analyses. Quantitative analysis of Claudin-2 and CTGF are shown above. ( D and E ) Quantitative analysis of α-SMA and collagen I in NRK49F cells are shown above. Data are expressed as mean data ± SEM (n = 3), ** P <0.01, NRK52E-si- Cldn2 -si-NC+NRK49F+NG; ## P <0.01, vs NRK52E-pcDNA3.1- Cldn2 -pcDNA3.1-NC+HG.

    Journal: Diabetes, Metabolic Syndrome and Obesity

    Article Title: Claudin-2 Mediates the Proximal Tubular Epithelial Cell–Fibroblast Crosstalk via Paracrine CTGF

    doi: 10.2147/DMSO.S432173

    Figure Lengend Snippet: Claudin-2 deficiency induced tubular epithelial CTGF facilitates fibroblasts to product matrix protein. ( A ) Western blot demonstrated CTGF inhibition in NRK52E-si- Cldn2 cells decreased α-SMA and collage I expression in NRK49F cells, when the cells were co-cultured in 5.5mM D-glucose (NG) medium. Overexpression of CTGF in NRK52E-pcDNA3.1- Cldn2 cells increased α-SMA and collage I expression in NRK49F cells, when the cells were co-cultured in 30mM D-glucose (HG) medium. ( B and C ) The interference efficiency of Claudin-2 and CTGF protein expression in NRK52E cells were confirmed by Western blot analyses. Quantitative analysis of Claudin-2 and CTGF are shown above. ( D and E ) Quantitative analysis of α-SMA and collagen I in NRK49F cells are shown above. Data are expressed as mean data ± SEM (n = 3), ** P <0.01, NRK52E-si- Cldn2 -si-NC+NRK49F+NG; ## P <0.01, vs NRK52E-pcDNA3.1- Cldn2 -pcDNA3.1-NC+HG.

    Article Snippet: The normal rat kidney fibroblast cell (NRK49F) and rat renal tubule epithelial cells (NRK52E) were obtained from ProCell Corporation (Wuhan, China) and cultured in RPMI-1640 medium (Corning, NY, USA).

    Techniques: Western Blot, Inhibition, Expressing, Cell Culture, Over Expression